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KMID : 0854720110310030199
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Korean Journal of Asthma, Allergy and Clinical Immunology
2011 Volume.31 No. 3 p.199 ~ p.206
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Anti-inflammatory and Anti-pruritic Effects of Portulaca oleracea L. Extract Using In Vitro and In Vivo Inflammation Model: LPS-treated Raw264.7 Cells, Keratinocytes, NC/Nga Mice and Hairless SKH-1 Mice
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Lim Yun-Young
Kim Hyeong-Mi
Park Won-Seok
Kim Jeong-Hwan
Shin Hong-Ju
Kim Myeung-Nam
Kim Beom-Joon
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Abstract
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Background: Portulaca oleracea L. is known to have detoxification and antibacterial effects. Inflammatory responses in LPS-treated Raw264.7 cells include the secretions of NO, PGE2 and numerous cytokines. Atopic dermatitis is a chronic inflammatory skin disease. This study was performed to identify the antiinflammatory or anti-pruritic effects of Portulaca oleracea L. extract in LPS-treated Raw264.7 cells and keratinocytes and the skin of NC/Nga mice with atopic dermatitis, as well as hairless mice with pruritus.
Methods: After the treatment of different concentrations for Portulaca oleracea L. extract in LPS-treated Raw264.7 cells and keratinocytes, the levels of NO, PGE2 and proinflammatory cytokines were measured in the media. Skin tissues of all Nc/Nga mice were subjected to H&E staining and toluidine blue staining. An antipruritic effect was defined as the number of scratching behaviors of the hairless SKH-1 mice treated with compound 48/80.
Results: Results: LPS- and IFN-gamma-treated Raw264.7 cells decreased NO and PGE2 productions compared to non-treated Raw264.7 cells. H&E or toluidine blue staining revealed that the thicknesses of the epidermis and dermis were reduced in NC/Nga mice with atopic dermatitis treated with Portulaca oleracea L. extract. However, Itching behaviors in hairless mice treated with Portulaca oleracea L. extract were not decreased compared to non-treated hairless mice.
Conclusion: The results of this study suggest that Portulaca oleracea L. extract could be a new therapeutic agent for atopic dermatitis.
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KEYWORD
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Portulaca oleracea L., Atopic dermatitis, Pruritus
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